xt mops running buffer (Bio-Rad)

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Bio-Rad xt mops running buffer
Xt Mops Running Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xt+mops+running+buffer/XT+MOPS+Running+Buffer/pm42045653-257-33-37
Average 95 stars, based on 181 article reviews

xt mops running buffer - by Bioz Stars, 2026-10

95/100 stars

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Nucleic Acid Electrophoresis:

Article Title: FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN)
Article Snippet: All protein samples were prepared by dilution with XT Sample Buffer (4×; Cat#1610791, Bio-Rad) and supplemented with XT Reducing Agent (20×; Cat#1610792, Bio-Rad). .. Gel electrophoresis was performed using 4–12% CriterionTM XT Bis-Tris Protein Gel (Cat#3450125, Bio-Rad) with XT MOPS Running Buffer (Cat#1610788, Bio-Rad). .. Proteins were transferred to a nitrocellulose membrane (BioRad Trans-Blot Turbo 0.2μm Nitrocellulose, #1704158 and 1704159) using a semi-dry transfer system (BioRad Trans-Blot Turbo Transfer System).

other:

Article Title: Systematic real-time profiling of Salmonella type III effector translocation provides quantitative resolution of the T3SS-1/T3SS-2 secretion dichotomy
Article Snippet: Precision Plus Protein All Blue Prestained Protein Standards (Bio-Rad, cat. no. 1610373) were used as molecular weight markers.

Article Title: Inosine misincorporation into mRNA triggers the integrated stress response and activates an innate immune gene expression signature
Article Snippet: Membranes were probed with anti-eIF2α (1:1000), anti-p-eIF2α (1:1000) (Cell Signaling Technology, 5324 and 3398), anti-PKR (1:1000), anti-p-PKR (1:1000) (AbCam, ab184257 and ab32036, respectively), anti- β-tubulin (1:5000) (Cell Signaling Technology, 2128) or anti-actin (1:1000) (Sigma A2066) overnight at 4°C.

Staining:

Article Title: Parallel Sequencing of the Two Arms on a Bispecific Antibody by Electron Capture Dissociation on a timsOmni Platform Reveals Several By-products in the Controlled Fab-arm Exchange Process
Article Snippet: For SDS-PAGE analyses 4 μg of antibody was mixed with XT sample buffer (Bio-Rad) with 50 mM DTT and PBS (total volume 20 μL), incubated at 95 °C on a thermo block for 10 min and loaded on a 12% Bis-Tris gel (Criterion XT). .. Gels were run in XT MOPS Running Buffer (Bio-Rad) for 15 min at 80 V, 30 min at 160 V, and finally 20 min at 180 V. Gel staining was performed using Precision Plus Protein Dual Color Standards (Bio-Rad) overnight, followed by destaining with Milli-Q water. .. Deglycosylated native antibodies were analyzed using an ultrahigh mass range (UHMR) Q-Exactive Orbitrap (Thermo Fisher Scientific).

Article Title: Parallel Sequencing of the Two Arms on a Bispecific Antibody by Electron Capture Dissociation on a timsOmni Platform Reveals Several By-products in the Controlled Fab-arm Exchange Process.
Article Snippet: For SDS-PAGE analyses 4 μg of antibody was mixed with XT sample buffer (Bio-Rad) with 50 mM DTT and PBS (total volume 20 μL), incubated at 95 °C on a thermo block for 10 min and loaded on a 12% Bis-Tris gel (Criterion XT). .. Gels were run in XT MOPS Running Buffer (Bio-Rad) for 15 min at 80 V, 30 min at 160 V, and finally 20 min at 180 V. Gel staining was performed using Precision Plus Protein Dual Color Standards (Bio-Rad) overnight, followed by destaining with Milli-Q water. ..

SDS Page:

Article Title: cAMP promotes acute lysosome biogenesis through TFEB nuclear import-export dynamics
Article Snippet: HeLa cells stably expressing TFEB-GFP were lysed, and TFEB-GFP was immunoprecipitated using GFP-Trap agarose beads (Chromotek, Cat. No. gta-20) following the manufacturer’s protocol. .. Eluted proteins were separated by SDS-PAGE using Criterion XT Pre-cast gels (Bio-Rad, Cat. No. 3450117) and XT MOPS running buffer (Bio-Rad, Cat. No. 1610788). ..

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